Root knot nematodes are among the most important groups of plant pests and is a major cause of crop loss. In this research, the control of nematode of tomato in greenhouse was conducted using tobacco dust as a bio-nematicide. I.M.P. diagnostic keys and some other common references were used to accurately identify species and the race of nematode. Experiments were carried out using complete randomized block design with 5 replication and 7 treatments at different rates of 0, 5, 10, 15, 20, 25 and 30 gr/kg. Soil shoot lengths, root fresh weights and root gall index were measured in all treatments. Results showed that 20gr. tobacco dust per kg soil was the best. The experiments were conducted over a two year period. The results showed that about 100gr per plant was the most effective treatment and the striped application at transplanting time proved to be recommendable.